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FADD Antibody (OTI1C11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71387

Novus Biologicals, part of Bio-Techne
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NBP2-71387
Conjugate
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human FADD (NP_003815) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FADD Antibody (OTI1C11) - Azide and BSA Free

Western Blot: FADD Antibody (OTI1C11)Azide and BSA Free [NBP2-71387]

Western Blot: FADD Antibody (OTI1C11)Azide and BSA Free [NBP2-71387]

Western Blot: FADD Antibody (OTI1C11) - Azide and BSA Free [NBP2-71387] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FADD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FADD.
Flow Cytometry: FADD Antibody (OTI1C11) - Azide and BSA Free [NBP2-71387]

Flow Cytometry: FADD Antibody (OTI1C11) - Azide and BSA Free [NBP2-71387]

Flow Cytometry: FADD Antibody (OTI1C11) - Azide and BSA Free [NBP2-71387] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FADD antibody, and then analyzed by flow cytometry.

Applications for FADD Antibody (OTI1C11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FADD

Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis.

Long Name

Fas-associated Death Domain Protein

Alternate Names

MORT1

Gene Symbol

FADD

Additional FADD Products

Product Documents for FADD Antibody (OTI1C11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FADD Antibody (OTI1C11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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