FGF basic/FGF2/bFGF Antibody (OTI3D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47749

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3D9

Concentration

1 mg/ml

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27007053). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (OTI3D9)

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9) [NBP1-47749]

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9) [NBP1-47749]

Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution
Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) [NBP1-47749]

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) [NBP1-47749]

Immunocytochemistry/Immunofluorescence: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.
Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (OTI3D9) [NBP1-47749]

Immunohistochemistry-Paraffin: FGF basic/FGF2/bFGF Antibody (OTI3D9) [NBP1-47749]

Immunohistochemistry-Paraffin: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.
View 1 more images

Applications for FGF basic/FGF2/bFGF Antibody (OTI3D9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Application Notes
For IHC-P: Heat-induced epitope retrieval with 10mM Sodium Citrate Buffer (pH 6.0 at 100C for 10min is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

BFGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human)

Gene Symbol

FGF2

UniProt

P09038

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (OTI3D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FGF basic/FGF2/bFGF Antibody (OTI3D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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