FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70718

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9)Azide and BSA Free [NBP2-70718]

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9)Azide and BSA Free [NBP2-70718]

Western Blot: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution
Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunocytochemistry/Immunofluorescence: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.
Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718]

Immunohistochemistry: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free [NBP2-70718] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.
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Applications for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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