Fyn Antibody (JE42-22)

Western Blot: Fyn Antibody (JE42-22) [NBP3-32357]

Western Blot: Fyn Antibody (JE42-22) [NBP3-32357] - Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (NBP3-32357) at 1/1,000 dilution.

Lane 1: Rat testis tissue lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Human brain tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 61 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32357) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.