Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30091
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, C. elegans, Drosophila, Mammal, Yeast
Applications
Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5E10-D8
Concentration
1 mg/ml
Product Summary for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)
Immunogen
Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]
Reactivity Notes
Predicted to react all mammals
Modification
Monomethyl Lys20
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)
Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.
Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.
Applications for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Dot Blot
1:2000
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Application Notes
This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP1-30091 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K20Me1
Gene Symbol
H4-16
UniProt
Additional Histone H4 Products
Product Documents for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)
Product Specific Notices for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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