Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30091CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, C. elegans, Drosophila, Mammal, Yeast
Applications
Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5E10-D8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]
Reactivity Notes
Predicted to react all mammals
Modification
Monomethyl Lys20
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [CoraFluor™ 1]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [CoraFluor™ 1]
Product Specific Notices for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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