Key Product Details

Species Reactivity

Human, Mouse, Rat, C. elegans, Drosophila, Mammal, Yeast

Applications

Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E10-D8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Histone H4 Antibodies »

Product Specifications

Immunogen

Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]

Reactivity Notes

Predicted to react all mammals

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H4 Antibody (5E10-D8) - Azide and BSA Free

Western Blot: Histone H4 Antibody (5E10-D8)Azide and BSA Free [NBP2-80771]

Western Blot: Histone H4 Antibody (5E10-D8)Azide and BSA Free [NBP2-80771]

Western Blot: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Analysis of Histone H4 meIK20 in HeLa histone lysates. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771]

Immunocytochemistry/ Immunofluorescence: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771]

Immunocytochemistry/Immunofluorescence: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue). Image from the standard format of this antibody.
Dot Blot: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20. Image from the standard format of this antibody.
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Applications for Histone H4 Antibody (5E10-D8) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Dot Blot

1:2000

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000
Application Notes
This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

H4Me1K20: Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 Antibody (5E10-D8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H4 Antibody (5E10-D8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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