Human 5-HT2A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11250
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1055217
Product Specifications
Immunogen
Synthetic peptide corresponding to the amino acids 1-75 in the N-term extracellular domain of the Serotonin Receptor 5-HT2A (HTR2A).
Accession # P28223
Accession # P28223
Specificity
Detects Human HTR2A in Direct ELISA. In flow cytometry, it detects Human HTR2A in HEK293 tranfected cells but it does not detect parental HEK293 or mock irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human 5-HT2A Antibody
Detection of Human 5-HT Transporter Compounds by Western Blot.
Western blot shows lysates of Human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human 5-HT Transporter Compounds Monoclonal Antibody (Catalog # MAB11250) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 5-HT Transporter Compounds at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of 5-HT Transporter Compounds in Transfected NSO (positive) and Wild Type NSO (negative).
5-HT Transporter Compounds was detected in immersion fixed transfected NSO (positive) and wild type NSO (negative) using Mouse Anti-Human 5-HT Transporter Compounds Monoclonal Antibody (Catalog # MAB11250) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of 5-HT2A in HEK293 Human Cell Line Transfected with Human 5-HT2A and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human 5-HT2A and eGFP was stained with (A) Mouse Anti-Human 5-HT2A Monoclonal Antibody (Catalog # MAB11250) or (B) Mouse IgG1 control antibody staining (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human 5-HT2A Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human 5-HT2A and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human 5-HT2A and eGFP
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Transfected NSO (positive) and Wild type NSO (negative)
Sample: Immersion fixed Transfected NSO (positive) and Wild type NSO (negative)
Western Blot
2 µg/mL
Sample: Human platelets
Sample: Human platelets
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5-HT2A
Long Name
5-Hydroxytryptamine Receptor 2A
Alternate Names
5HT2A, HTR2A
Gene Symbol
HTR2A
UniProt
Additional 5-HT2A Products
Product Documents for Human 5-HT2A Antibody
Product Specific Notices for Human 5-HT2A Antibody
For research use only
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