Human Angiopoietin-like Protein 3/ANGPTL3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB38292
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 388610
Product Summary for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin‑like Protein 3/ANGPTL3
Ile19-Glu460
Accession # Q9Y5C1
Ile19-Glu460
Accession # Q9Y5C1
Specificity
Detects human Angiopoietin‑like Protein 3/ANGPTL3 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody
Detection of Human Angiopoietin‑like Protein 3/ANGPTL3 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line (negative control), HepG2 human hepatocellular carcinoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Angiopoietin-like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38292) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 3/ANGPTL3
Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that belongs to the angiopoietin family. It is expressed in the liver and circulates as 70 kDa, 50 kDa, and 32 kDa species. These include full length ANGPTL3 as well as the proteolytically separated N- and C-terminal fragments which contain a coiled coil domain and fibrinogen-like domain, respectively. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. Mature human ANGPTL3 shares 77% amino acid sequence identity with mouse ANGPTL3.
Alternate Names
AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3
Gene Symbol
ANGPTL3
UniProt
Additional Angiopoietin-like Protein 3/ANGPTL3 Products
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Product Documents for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody
Product Specific Notices for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody
For research use only
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