Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Apolipoprotein A‑I/ApoA1 in HepG2 Human Cell Line by Flow Cytometry

HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Apolipoprotein A‑I/ApoA1 in Human Hepatocytes.

Apolipoprotein A-I/ApoA1 was detected in immersion fixed human hepatocytes using Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human Apolipoprotein A-I/ApoA1 Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and human hepatocytes

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

0.2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-I/ApoA1

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Entrez Gene IDs

335 (Human); 11806 (Mouse); 25081 (Rat)

Gene Symbol

APOA1

UniProt

P02647

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Human Apolipoprotein A-I/ApoA1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Apolipoprotein A-I/ApoA1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Apolipoprotein A-I/ApoA1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB36641

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Apolipoprotein A-I/ApoA1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Apolipoprotein A-I/ApoA1 Antibody

Detection of Human Apolipoprotein A‑I/ ApoA1 by Western Blot.