Human Caspase-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB62156
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2666E
Product Summary for Human Caspase-1 Antibody
Immunogen
E. coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466
Asn120-Asp297
Accession # P29466
Specificity
Detects human Caspase-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Caspase-1 Antibody
Detection of Human Caspase-1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62156) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Caspase-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Caspase-1 in PC-3 Human Cell Line.
Caspase-1 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and U937 human histiocytic lymphoma cell line (negative control) using Rabbit Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62156) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Caspase-1 in Human Lymph Node.
Caspase-1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Rabbit Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62156) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Caspase-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line
Sample: Immersion fixed PC-3 human prostate cancer cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Western Blot
2 µg/mL
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-1
Caspase-1, also known as IL-1 beta-Converting Enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.
Additional Caspase-1 Products
Product Documents for Human Caspase-1 Antibody
Product Specific Notices for Human Caspase-1 Antibody
For research use only
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