Human Caspase-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6215
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Primate - Cercopithecus aethiops (African Green Monkey), Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 661228
Product Specifications
Immunogen
E.coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466
Asn120-Asp297
Accession # P29466
Specificity
Detects human Caspase-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Caspase-1 Antibody
Detection of Human, Mouse, and Rat Caspase‑1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Caspase‑1 in THP‑1 Human Cell Line.
Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Caspase-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using MAB6215 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-1
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.
Additional Caspase-1 Products
Product Documents for Human Caspase-1 Antibody
Product Specific Notices for Human Caspase-1 Antibody
For research use only
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