Human CCL26/Eotaxin-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6531
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2152H
Product Specifications
Immunogen
E. coli-derived recombinant human CCL26/Eotaxin-3
Thr24-Leu94
Accession # Q9Y258
Thr24-Leu94
Accession # Q9Y258
Specificity
Detects human CCL26/Eotaxin-3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CCL26/Eotaxin-3 Antibody
Detection of Human CCL26/Eotaxin‑3 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB6531) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CCL26/Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CCL26/Eotaxin-3 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human IL‑4 (Catalog # 204-IL)
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human IL‑4 (Catalog # 204-IL)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL26/Eotaxin-3
Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin‑3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.
Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.
References
- Gou, R.F. et al. (1999) Genomics 58:313.
- Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
- Shinkai, A. et al. (1999) J. Immunol. 163:1602.
Alternate Names
Eotaxin-3, Eotaxin3, SCYA26
Entrez Gene IDs
10344 (Human)
Gene Symbol
CCL26
UniProt
Additional CCL26/Eotaxin-3 Products
Product Documents for Human CCL26/Eotaxin-3 Antibody
Product Specific Notices for Human CCL26/Eotaxin-3 Antibody
For research use only
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