Human CCL26/Eotaxin‑3 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF346
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, Immunohistochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL26/Eotaxin-3 (R&D Systems, Catalog # 346-E3)
Thr24-Leu94
Accession # Q9Y258
Thr24-Leu94
Accession # Q9Y258
Specificity
Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) Eotaxin, rhEotaxin‑2, recombinant mouse (rm) Eotaxin, and rmEotaxin‑2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human CCL26/Eotaxin‑3 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346-E3)
Sample: Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346-E3)
Human CCL26/Eotaxin-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using BAF346 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL26/Eotaxin-3
Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4. Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands.
References
- Gou, R.F. et al. (1999) Genomics 58:313.
- Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
- Shinkai, A. et al. (1999) J. Immunol. 163:1602.
Alternate Names
Eotaxin-3, Eotaxin3, SCYA26
Entrez Gene IDs
10344 (Human)
Gene Symbol
CCL26
UniProt
Additional CCL26/Eotaxin-3 Products
Product Documents for Human CCL26/Eotaxin‑3 Biotinylated Antibody
Product Specific Notices for Human CCL26/Eotaxin‑3 Biotinylated Antibody
For research use only
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