Human CD20 Peptide. The epitope recognized by rituximab
Binder M, et al. (2006) 108:1975-8. doi: 10.1182/blood-2006-04-014639. Epub 2006 May 16. PMID: 16705086

Specificity

Detects human CD20 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CD20 Antibody

Detection of CD20 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™

CD20 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD20 Monoclonal Antibody (MAB42252) at 3ug/mL dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of B-cells. Protocol available in COMET™ Panel Builder.

Detection of Human CD20 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD20 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD20 in Human PBMCs by Flow Cytometry.

Human PBMCs were stained with (A) Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) or (B) Rabbit IgG Isotype Control Antibody (MAB1050), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Applications for Human CD20 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMC

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Multiplex Immunofluorescence

3 µg/mL
Sample: Immersion fixed paraffin embedded sections of human tonsil

Simple Western

25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for Human CD20 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human CD20 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD20 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB42252

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen