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Human CD4 Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3791T

R&D Systems, part of Bio-Techne
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FAB3791T-025
FAB3791T-100

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 11830

Product Specifications

Immunogen

Recombinant human CD4
Extracellular domain
Accession # P01730

Specificity

Detects human CD4 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse CD4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD4 Alexa Fluor® 594-conjugated Antibody

Detection of CD4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB3791T) or (B) Mouse IgG2AAlexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD4 Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a 54-60 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed predominantly on hematopoietic cells such as T cells, monocytes and Langerhans cells. T cells known to be positive for CD4 include Th1, Th2, Th17, Th22, Th9, Tfh and Treg subsets, plus select thymocytes. CD4 exists as either an oxidized monomer (3 internal disulfide bonds), a reduced monomer (2 internal disulfide bonds) and a disulfide-linked dimer. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. In particular, CD4 in its dimeric form binds to the MHC complex at non-polymorphic sites. Monomeric CD4 has also been shown to be a coreceptor for HIV entry and specifically to bind gp120, the external envelope glycoprotein of HIV. Over amino acids (aa) 26-396 (the extracellular domain), human and mouse CD4 share 58% aa sequence identity.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

Additional CD4 Products

Product Documents for Human CD4 Alexa Fluor® 594-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD4 Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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