Human CD8 alpha Antibody

Detection of CD8 alpha in Hodgkins Lymphoma via Multiplex Immunofluorescence staining on COMET™

CD8 alpha was detected in immersion fixed paraffin-embedded sections of Hodgkins Lymphoma using Mouse Anti-Human CD8 alpha Monoclonal Antibody (MAB3801) at 15 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Protocol available in COMET™ Panel Builder.

CD8 alpha in Human Tonsil.

CD8a was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB3801) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.