Human cIAP-2/HIAP-1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF8171
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human cIAP‑2/HIAP‑1
Asn2-Ser604
Accession # U45878
Asn2-Ser604
Accession # U45878
Specificity
Detects human cIAP‑2/HIAP‑1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human cIAP-2/HIAP-1 Biotinylated Antibody
Detection of Human cIAP‑2/HIAP‑1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8171) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for cIAP-2/HIAP-1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human cIAP-2/HIAP-1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-2/HIAP-1
cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
References
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
- Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
Alternate Names
BIRC3, cIAP2, HIAP-1, MIHC
Entrez Gene IDs
330 (Human)
Gene Symbol
BIRC3
UniProt
Additional cIAP-2/HIAP-1 Products
Product Documents for Human cIAP-2/HIAP-1 Biotinylated Antibody
Product Specific Notices for Human cIAP-2/HIAP-1 Biotinylated Antibody
For research use only
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