Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CFHR1, rhCFHR2, or rhCFHR5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Complement Factor H-related 4/CFHR4 Antibody

Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H-related 4/ CFHR4 at approximately 45 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Complement Factor H-related 4/CFHR4 in Human PBMCs.

Complement Factor H-related 4/CFHR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMC) using Human Complement Factor H-related 4 Monoclonal Antibody (Catalog # MAB5980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Complement Factor H-related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.

Applications for Human Complement Factor H-related 4/CFHR4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMC)

Intracellular Staining by Flow Cytometry

0.25 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line cells fixed with paraformaldehyde and permeabilized with saponin

Western Blot

2 µg/mL
Sample: Human liver tissue under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using MAB5980 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 4/CFHR4

FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.

Alternate Names

CFHL4, CFHR4, Complement Factor Hrelated 4, FHR4

Entrez Gene IDs

10877 (Human)

Gene Symbol

CFHR4

UniProt

Q92496

Additional Complement Factor H-related 4/CFHR4 Products

Product Documents for Human Complement Factor H-related 4/CFHR4 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Complement Factor H-related 4/CFHR4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Complement Factor H-related 4/CFHR4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5980

Key Product Details

Species Reactivity

Validated:

Cited:

Applications