Human CXCR2/IL-8RB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB331
Clone 48311 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Guinea Pig, Rabbit
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization
Cited:
Bioassay, ELISA Development, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 48311
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880
Met1-Leu355
Accession # AAB25880
Specificity
Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCR2/IL-8RB Antibody
Detection of CXCR2/IL‑8 RB in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
CXCR2/IL-8 RB in Human Lymph Node.
CXCR2/IL-8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL-8 RB Antibody.
Recombinant Human CXCL1/GROa (Catalog # 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GROa (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331). The ND50 is typically 1-5 µg/mL.Applications for Human CXCR2/IL-8RB Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Neutralization
Measured by its ability to neutralize CXCL1/GRO alpha/KC/CINC-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CXCL1/GRO alpha/KC/CINC-1.
Reviewed Applications
Read 6 reviews rated 4.3 using MAB331 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR2/IL-8RB
Long Name
Interleukin 8 Receptor B
Alternate Names
CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB
Gene Symbol
CXCR2
UniProt
Additional CXCR2/IL-8RB Products
Product Documents for Human CXCR2/IL-8RB Antibody
Product Specific Notices for Human CXCR2/IL-8RB Antibody
For research use only
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