Human DcR3/TNFRSF6B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF142
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DcR3/TNFRSF6B
Val24-His300
Accession # O95407
Val24-His300
Accession # O95407
Specificity
Detects human DcR3/TNFRSF6B in ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, and rhTRAIL R4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human DcR3/TNFRSF6B Antibody
DcR3/TNFRSF6B in Human Stomach Cancer Tissue.
DcR3/TNFRSF6B was detected in immersion fixed paraffin-embedded sections of human stomach cancer using 10 µg/mL Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody.
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142). The ND50 is typically 0.1-0.5 µg/mL.Applications for Human DcR3/TNFRSF6B Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach cancer subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human stomach cancer subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC)
Sample: Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC)
Neutralization
Measured by its ability to neutralize DcR3/TNFRSF6B-mediated inhibition of apoptosis in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 50 ng/mL Recombinant Human DcR3/TNFRSF6B Fc Chimera, 2 ng/mL Recombinant Human Fas Ligand/TNFSF6.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DcR3/TNFRSF6B
Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6, and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNF RII (29%), and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2, and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Over-expression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.
References
- Pitti, R.M. et al. (1998) Nature 396:699.
- Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
- Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1230.
Long Name
Decoy Receptor 3
Alternate Names
M68, TNFRSF6B, TR6
Gene Symbol
TNFRSF6B
UniProt
Additional DcR3/TNFRSF6B Products
Product Documents for Human DcR3/TNFRSF6B Antibody
Product Specific Notices for Human DcR3/TNFRSF6B Antibody
For research use only
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