Human DSCAM Long Isoform Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3666
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DSCAM long isoform
Glu18-Met1595
Accession # O60469
Glu18-Met1595
Accession # O60469
Specificity
Detects human DSCAM in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DSCAM-L1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DSCAM Long Isoform Antibody
Detection of Recombinant Human DSCAM by Western Blot.
Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM-L1 (Catalog # 3315-DL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
DSCAM in A172 Human Cell Line.
DSCAM was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse Human DSCAM Long Isoform Antibody by Western Blot
The C-terminus of DSCAM is not required for protein stability or localization. (C) Western blots of DSCAM protein immunoprecipitated from neonatal brains showed no change in the size or amount of DSCAM in Dscam∆C/∆C mutants. The antibody specificity is confirmed by the lack of signal from Dscam-/- brains. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27637097), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human DSCAM Long Isoform Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line
Sample: Immersion fixed A172 human glioblastoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human DSCAM (Catalog # 3666-DS)
Sample: Recombinant Human DSCAM (Catalog # 3666-DS)
Reviewed Applications
Read 1 review rated 3 using AF3666 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DSCAM
Human DSCAM is a member of the Ig superfamily that maps to a region in chromosome 21 associated with Down Syndrome mental retardation. It encodes a 230 kDa type I membrane protein with a large extracellular domain comprising ten C2 type Ig like domains and six fibronectin type III repeats. The intracellular domain of human DSCAM does not share significant sequence homology with Drosophila DSCAM. Human DSCAM is expressed in axonal and dendritic processes of the central and peripheral nervous system. It is involved in hemophilia interactions and has been implicated in neurogenesis. By alternative splicing, a soluble form of human DSCAM that is identical to the transmembrane form through residue 1571 has been described. The extracellular domain of human and mouse DSCAM share 98% amino acid sequence homology.
Long Name
Down Syndrome Cell Adhesion Molecule
Alternate Names
CHD2-42, CHD2-52
Gene Symbol
DSCAM
UniProt
Additional DSCAM Products
Product Documents for Human DSCAM Long Isoform Antibody
Product Specific Notices for Human DSCAM Long Isoform Antibody
For research use only
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