Human ERK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1940
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Human ERK1 synthetic peptide
Met1-Val28
Accession # P27361
Met1-Val28
Accession # P27361
Specificity
Detects human ERK1 in Western blots. Because the peptide immunogen corresponds to a region of ERK1 largely absent in ERK2, no reactivity with ERK2 is present.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human ERK1 Antibody
Detection of Human ERK1 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1940) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog # 1879-KS) and Recom-binant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ERK1 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line
Sample: MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK1
Long Name
Extracellular Signal-regulated Kinase 1
Alternate Names
MAPK3, P44ERK1, p44mapk, PRKM3
Gene Symbol
MAPK3
UniProt
Additional ERK1 Products
Product Documents for Human ERK1 Antibody
Product Specific Notices for Human ERK1 Antibody
For research use only
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