Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FANCD2 in Human Tonsil.

FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of FANCD2 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human FANCD2 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for FANCD2 at approximately 152 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human FANCD2 Specificity Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of MMP-9 in Human Tonsil.

Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for MMP9 mRNA (ACD RNAScope Probe, catalog #311338; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human MMP9 monoclonal antibody (R&D Systems catalog # MAB936) at 15ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes.

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Applications for Human FANCD2 Antibody

Application

Recommended Usage

Dual RNAscope ISH-IHC Compatible

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

FANCD2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FANCD2 knockout HeLa cell line.

Simple Western

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and U2OS human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FANCD2

Fanconi anemia group D2 protein, encoded by the FANCD2 gene, is a member of the Fanconi anemia complementation (FANC) group that also includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCE, FANCF, FANCG, and FANCL. The members of the Fanconi anemia complementation group share little sequence similarities.

Long Name

Fanconi Anemia, Complementation Group D2

Alternate Names

FA4, FACD, FAD, FAD2, FANCD

Entrez Gene IDs

2177 (Human); 211651 (Mouse); 312641 (Rat)

Gene Symbol

FANCD2

UniProt

Q9BXW9

Additional FANCD2 Products

Product Documents for Human FANCD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FANCD2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FANCD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9369

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all FANCD2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FANCD2 Antibody

Detection of Human FANCD2 by Western Blot.