Human Fibroblast Activation Protein alpha/FAP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3715
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Dual RNAscope ISH-IHC Compatible, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot
Cited:
CAR-T (Flow Cytometry), CAR-T (Flow Cytomety), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Photoimmunotherapy, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha/FAP
Leu26-Asp760
Accession # Q12884
Leu26-Asp760
Accession # Q12884
Specificity
Detects human Fibroblast Activation Protein alpha/FAP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Fibroblast Activation Protein alpha/FAP Antibody
Detection of Human Fibroblast Activation Protein alpha/FAP by Western Blot.
Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Fibroblast Activation Protein alpha/FAP in Human Squamous Cell Carcinoma.
Fibroblast Activation Protein a/FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein a/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Fibroblast Activation Protein alpha/FAP in Human Basal Cell Carcinoma.
Fibroblast Activation Protein alpha/FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human Fibroblast Activation Protein alpha/FAP Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human esophagus
Sample: Immersion fixed paraffin-embedded sections of human esophagus
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma and human basal cell carcinoma
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma and human basal cell carcinoma
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Fibroblast Activation Protein alpha/FAP (Catalog # 3715-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Fibroblast Activation Protein alpha/FAP (Catalog # 3715-SE), see our available Western blot detection antibodies
Knockout Validated
Fibroblast
Activation Protein alpha/FAP is specifically detected in
WI‑38 human lung fibroblast parental cell line but is not
detectable in Fibroblast Activation Protein alpha/FAP knockout
WI‑38 human lung fibroblast cell line.
Simple Western
10 µg/mL
Sample: IMR‑90 human lung fibroblast cell line and WI‑38 human lung fibroblast cell line
Sample: IMR‑90 human lung fibroblast cell line and WI‑38 human lung fibroblast cell line
Western Blot
0.5 µg/mL
Sample: WI‑38 human lung fibroblast cell line
Sample: WI‑38 human lung fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.5 using AF3715 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
References
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Alternate Names
FAP, Seprase
Gene Symbol
FAP
UniProt
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Product Documents for Human Fibroblast Activation Protein alpha/FAP Antibody
Product Specific Notices for Human Fibroblast Activation Protein alpha/FAP Antibody
For research use only
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