Human HLA-DR Antibody

Detection of HLA-DRA in Human Tonsil via Multiplex Immunofluorescence staining on COMET™​

HLA-DRA was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 1 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​

Detection of Human HLA-DR by Western Blot.

Western Blot shows lysates of HDLM-2 human Hodgkin’s lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA-DR Monoclonal Antibody (Catalog # MAB11555) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA-DR at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of HLA-DR in Human Tonsil.

HLA-DR was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-DR Monoclonal Antibody (Catalog # MAB11555) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.