Western blot shows lysates of HEK293 human embryonic kidney cell line either mock-transfected or transfected with human IGDCC3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGDCC3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IGDCC3 in HEK293 Human Cell Line Transfected with Human IGDCC3 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human IGDCC3 and eGFP was stained with either (A) Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

IGDCC3 in HepG2 Human Cell Line.

IGDCC3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human IGDCC3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human IGDCC3 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human IGDCC3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGDCC3

IGDCC3 (Immunoglobulin superfamily DCC subclass member 3), also known as Punc, is 814 aa single-pass type membrane protein and shares 84% aa identity with mouse IGDCC3. It is a member of the neural cell adhesion molecule family and contains two fibronectin type III repeats and four immunoglobulin-like domains. IDGCC3 is high expressed in the brain and has been suggested to play a role in motor coordination. It has also been suggested to play a role in breast cancer.

Long Name

Immunoglobulin Superfamily, DCC Subclass, Member 3

Alternate Names

HsT18880, PUNC

Entrez Gene IDs

9543 (Human); 19289 (Mouse)

Gene Symbol

IGDCC3

UniProt

Q81VU1

Additional IGDCC3 Products

Product Documents for Human IGDCC3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IGDCC3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IGDCC3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8559

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IGDCC3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IGDCC3 Antibody

Detection of Human IGDCC3 by Western Blot.