Human IL-18 R alpha/IL-1 R5 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB840G
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Glu20-Arg329
Accession # Q13478
Specificity
Clonality
Host
Isotype
Scientific Data Images
Detection of IL-18 R alpha/IL-1 R5 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 5 µg/mL PHA for 48 hours were stained with Mouse Anti-Human IL-18 Ra/IL-1 R5 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB840G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications
Flow Cytometry
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and PHA
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18 R alpha/IL-1 R5
Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
References
- Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-18 R alpha/IL-1 R5 Products
Product Specific Notices
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