Human Integrin alpha3/CD49c Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1345
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # IA3
Product Specifications
Immunogen
Human milk epithelial cell line
Specificity
Detects human Integrin alpha3/CD49c.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin alpha3/CD49c Biotinylated Antibody
Integrin alpha3/CD49c in HT1080 Human Cell Line.
Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Biotinylated Monoclonal Antibody (Catalog # BAM1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin alpha3/CD49c Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HT1080 human fibrosarcoma cell line
Sample: HT1080 human fibrosarcoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line
Sample: Immersion fixed HT1080 human fibrosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 3/CD49c
Additional Integrin alpha 3/CD49c Products
Product Documents for Human Integrin alpha3/CD49c Biotinylated Antibody
Product Specific Notices for Human Integrin alpha3/CD49c Biotinylated Antibody
For research use only
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