HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a3/CD49c Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1345F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha3/CD49c Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HT1080 human fibrosarcoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 3/CD49c

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta1 subfamily, of cell membrane adhesion molecules (1 - 3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha- and beta-subunit (1). VLA-3 is composed of an alpha3 and beta1 subunit. The alpha3/CD49c subunit is a 130 - 150 kDa type I TM glycoprotein. It only associates with the beta1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).

References

Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Alternate Names

CD49c, ITGA3

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Gene Symbol

ITGA3

Additional Integrin alpha 3/CD49c Products

Product Documents for Human Integrin alpha3/CD49c Fluorescein-conjugated Antibody

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COA

SDS

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Product Specific Notices for Human Integrin alpha3/CD49c Fluorescein-conjugated Antibody

For research use only

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Flow Cytometry

Human Integrin alpha3/CD49c Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1345F

Key Product Details

Species Reactivity

Validated:

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Applications

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alpha3/CD49c Fluorescein-conjugated Antibody

Detection of Integrin alpha3/CD49c in HT1080 Human Cell Line by Flow Cytometry.