Integrin alpha4/CD49d was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin alpha4/CD49d Biotinylated Monoclonal Antibody (Catalog # BAM1354) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin alpha4/CD49d Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta1 (CD29) or beta7. Integrins alpha4 beta1 and alpha4 beta7 are receptors for fibronectin and VCAM. Integrin alpha4 beta1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Alternate Names

CD49d, ITGA4, VLA-4

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Human Integrin alpha4/CD49d Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Integrin alpha4/CD49d Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alpha4/CD49d Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1354

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alpha4/CD49d Biotinylated Antibody

Integrin alpha4/CD49d in MOLT-4 Human Cell Line.