Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Integrin alpha4/CD49d in U937 Human Cell Line.

Integrin a4/ CD49d was detected in immersion fixed U937 human histiocytic lymphoma cell line using 10 µg/mL Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin alpha4/CD49d Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta1 (CD29) or beta7. Integrins alpha4 beta1 and alpha4 beta7 are receptors for fibronectin and VCAM. Integrin alpha4 beta1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Alternate Names

CD49d, ITGA4, VLA-4

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Human Integrin alpha4/CD49d Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin alpha4/CD49d Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alpha4/CD49d Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1354

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alpha4/CD49d Antibody

Detection of Integrin alpha4/CD49d in Human Blood Lymphocytes by Flow Cytometry.