Human Integrin alpha4/CD49d Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1354
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Cited:
Bioassay, Flow Cytometry, Immunocytochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7.2R
Product Specifications
Immunogen
DX-3 human melanoma cell line
Specificity
Detects human Integrin alpha4/CD49d. Recognizes the human Integrin alpha4/CD49d subunit.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin alpha4/CD49d Antibody
Detection of Integrin alpha4/CD49d in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Integrin alpha4/CD49d in U937 Human Cell Line.
Integrin a4/ CD49d was detected in immersion fixed U937 human histiocytic lymphoma cell line using 10 µg/mL Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin alpha4/CD49d Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 4/CD49d
The alpha4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta1 (CD29) or beta7. Integrins alpha4 beta1 and alpha4 beta7 are receptors for fibronectin and VCAM. Integrin alpha4 beta1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).
Alternate Names
CD49d, ITGA4, VLA-4
Gene Symbol
ITGA4
Additional Integrin alpha 4/CD49d Products
Product Documents for Human Integrin alpha4/CD49d Antibody
Product Specific Notices for Human Integrin alpha4/CD49d Antibody
For research use only
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