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Human LDLR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2148

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

ELISA Capture, ELISA Development, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 472413

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LDLR
Ala22-Arg788
Accession # P01130

Specificity

Detects human LDLR in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDLR, recombinant human LRP-5, or rmLRP-6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LDLR Antibody

Detection of LDL R antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of LDLR in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B).

Detection of LDLR in A172 cells by Flow Cytometry.

A172 cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Detection of LDLR in U-118-MG cells by Flow Cytometry

U-118-MG cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LDLR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line, A172 human glioblastoma cell line, and U-118-MG human glioblastoma/astrocytoma cell line

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human LDLR (Catalog # 2148‑LD), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human LDLR (Catalog # 2148-LD) under non-reducing conditions only

Human LDLR Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human LDLR Biotinylated Antibody (Catalog # BAF2148)
  • Standard: Recombinant Human LDLR Protein (Catalog # 2148-LD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB2148 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LDLR

The Low Density Lipoprotein Receptor (LDLR) is the founding member of the LDLR family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta-propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDLR is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta-propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDLR is 51% aa identical to the extracellular region of human VLDLR, and 79% aa identical to the extracellular region of mouse LDLR. LDLR is constitutively expressed and binds ApoB of LDL and ApoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia (6).

References

  1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
  2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
  3. Yamamoto, T. et al. (1984) Cell 39:27.
  4. Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
  5. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
  6. Varret, M. et al. (2008) Clin Genet. 73:1.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Entrez Gene IDs

3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)

Gene Symbol

LDLR

UniProt

Additional LDLR Products

Product Documents for Human LDLR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LDLR Antibody

For research use only

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