Human LYVE-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB20892

R&D Systems, part of Bio-Techne
Discontinued Product
MAB20892 has been discontinued. View all LYVE-1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 537028

View all LYVE-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LYVE-1
Ser24-Thr238
Accession # Q9Y5Y7

Specificity

Detects endogenous human LYVE-1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LYVE-1 Antibody

Detection of Human LYVE-1 antibody by Western Blot.

Detection of Human LYVE-1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LYVE-1 Monoclonal Antibody (Catalog # MAB20892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LYVE-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LYVE-1 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of LYVE-1 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human LYVE-1 Monoclonal Antibody (Catalog # MAB20892, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of LYVE-1 antibody in Human PBMC antibody by Flow Cytometry.

Detection of LYVE-1 in Human PBMC by Flow Cytometry.

Human PBMC were cultured with 50 ng/ml Recombinant Human M-CSF (Catalog # 216-MC) for 10 days (see Volk-Draper et al (2017) PLoS One 12(6): e0179257), then stained with either (A) Mouse Anti-Human LYVE-1 Monoclonal Antibody (Catalog # MAB20892) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LYVE-1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells, and M-CSF-treated Human PBMC

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and 293T human embryonic kidney cell line

Reviewed Applications

Read 5 reviews rated 3.8 using MAB20892 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1 ‑ 3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9 ‑11).

References

  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
  7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Long Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Alternate Names

LYVE1, XLKD1

Entrez Gene IDs

10894 (Human); 114332 (Mouse); 293186 (Rat)

Gene Symbol

LYVE1

UniProt

Q9Y5Y7

Additional LYVE-1 Products

Product Documents for Human LYVE-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LYVE-1 Antibody

For research use only

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