Human Mcl-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB828
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Knockout Validated, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 542808
Product Specifications
Immunogen
E. coli-derived recombinant human Mcl-1
Met1-Gly230
Accession # Q07820
Met1-Gly230
Accession # Q07820
Specificity
Detects human Mcl-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Mcl-1 Antibody
Detection of Human Mcl-1 by Western Blot.
Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mcl-1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot Shows Human Mcl‑1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Mcl-1 knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mcl-1 at approximately 40 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Mcl-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Knockout Validated
Mcl‑1
is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in
Mcl‑1 knockout A431 cell line.
Western Blot
1 µg/mL
Sample: HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line
Sample: HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB828 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mcl-1
Long Name
Myeloid Cell Leukemia Sequence 1
Alternate Names
BCL2L3, Mcl1
Gene Symbol
MCL1
UniProt
Additional Mcl-1 Products
Product Documents for Human Mcl-1 Antibody
Product Specific Notices for Human Mcl-1 Antibody
For research use only
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