Human/Mouse E-Cadherin Antibody

E-Cadherin and SOX2 in BG01V Human Stem Cells.

E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

E‑Cadherin in Human Stomach.

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human and Mouse E‑Cadherin by Simple Western^TM.

Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E-Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

E-Cadherin in Human Stomach Using Dual RNAscope^®ISH and IHC.

E-Cadherin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human stomach probed with ACD RNAScope^®Probe (Catalog # 311091) and stained using ACD RNAscope^®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Detection of Human E-Cadherin by Immunocytochemistry/Immunofluorescence

Impact of H. pylori infection on ES cell-derived gastric organoids. (a) HE staining of ES-cell derived gastric organoids infected with H. pylori for 12 hours. Scale bar: 200 µm. (b) Immunostaining of gastric organoids infected with H. pylori for 12 hours. “No infection” indicates that the organoid was injected with bacteria-free Brucella Broth. HP, H. pylori; E-cad, E-cadherin. Scale bar: 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30374120), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human E-Cadherin by Immunocytochemistry/Immunofluorescence

Development and Characterization of an iPSC-Derived Fallopian Tube Organoid. (a) Schematic of factors involved in the differentiation of fallopian tube organoids. (b) Bright field image and H&E staining of FTE organoid at day 14. (c–e) Immunocytochemistry for FTE markers TUBB4A, FOXJ1 and PAX8 and epithelial marker CDH1 (E-Cadherin) at organoid culture day 14. (f) Immunocytochemistry for FTE markers PAX8, TUBB4A, OVGP1 and epithelial marker CDH1 at FTE organoid culture day 45, along with human fallopian tube tissue. (g) Immunocytochemistry for FTE markers TUBB4A and PAX8 at FTE organoid culture day 45. (h) Gene expression of fallopian tube markers OVGP1 (for 2 different primers), FOXJ1, TNFaIP2 and PAX8, as well as kidney markers SALL1 and FOXD1 at organoids culture day 45, human fallopian tube and kidney. The color matrix of the heat map represents the log2(Ratio) of each individual gene relative to its expression at the iPSC stage. Relative gene expression to iPSC stage (day 0) was calculated using delta deltaCt method and normalized to endogenous GAPDH level for 87iCTR-n3 iPSC line (i) H&E staining of FTE organoid at culture day 45 and day 180, and human fallopian tube tissue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28878359), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse E-Cadherin by Immunohistochemistry

Immunohistological visualization of engineered EcN strains in tissue sections.These sections are taken from proximal colons of mice receiving different bacteria. Sectioning protocol was designed to preserve mucus and luminal content. Sections were stained with fluorescently labeled antibodies: anti-E-cadherin (green), anti-Muc2 (red), and anti-LPS (blue). The first column shows bright-field images of the sections. The last column shows an overlay of all stains. The white dotted lines represent the boundary of the epithelium and mucus layers. The leftmost parts represent the epithelium, the center parts represent the mucus layers and the rightmost parts represent the lumen (scale bar = 100 μm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31811125), licensed under a CC-BY license. Not internally tested by R&D Systems.