Detects mouse Frizzled-1 in direct ELISAs and Western blots. Detects human Frizzled-1 in Flow Cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human/Mouse Frizzled-1 Antibody

Detection of Frizzled-1 in HEK293 Human Cell Line transfected with Human Frizzled-1 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with Frizzled-1 was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Detection of Frizzled-1 in HEK293 Human Cell Line by Flow Cytometry.

HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Detection of Frizzled-1 in NS0 cell line transfected with mouse Frizzled-1 by Flow Cytometry

NS0 cell line transfected with mouse Frizzled-1 were stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Frizzled-1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: see below

Western Blot

1 µg/mL
Sample: Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-1

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD comprises 642 amino acids and shares 95% identity with the human orthologue. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta-catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).

References

Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
Hsieh, J-C. (2004) Front. Biosci., 9:1333.
Xu, L. et al. (2001) Matrix Biol. 2:147.
Tamai, K. et al. (2000) Nature 407:530.
Pinson, K. et al. (2000) Nature 407:535.
Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.

Alternate Names

Frizzled1, FZD1

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Gene Symbol

FZD1

UniProt

O70421

Additional Frizzled-1 Products

Product Documents for Human/Mouse Frizzled-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Frizzled-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry