Human/Mouse GATA-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6330
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Immunohistochemistry, Multiplex Immunofluorescence, Simple Western, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 634913
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Pro135-Ser258
Accession # P23771
Specificity
Detects human and mouse GATA-3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse GATA-3 Antibody
Detection of GATA-3 in Human Breast Cancer via Multiplex Immunofluorescence staining on COMET™
GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer using Mouse Anti-Human GATA-3 Monoclonal Antibody (MAB6330) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus with dim cytoplasmic background signal. Protocol available in COMET™ Panel Builder.
Detection of Human and Mouse GATA‑3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Moue Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for full length (FL) GATA-3 at approximately 52 kDa and the splice form (SF) found in MCF-7 cells at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GATA‑3 in MCF‑7 Human Cell Line.
GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human/Mouse GATA-3 Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Multiplex Immunofluorescence
20 µg/mL
Sample: Paraffin embedded tissue sections of Human Breast Cancer
Sample: Paraffin embedded tissue sections of Human Breast Cancer
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line
Sample: Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line
Reviewed Applications
Read 1 review rated 4 using MAB6330 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-3
Additional GATA-3 Products
Product Documents for Human/Mouse GATA-3 Antibody
Product Specific Notices for Human/Mouse GATA-3 Antibody
For research use only
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