Human/Mouse/Rat B-Raf Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3424
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 336016
Product Summary for Human/Mouse/Rat B-Raf Antibody
Immunogen
E. coli-derived recombinant human B-Raf
Met1-His766
Accession # P15056
Met1-His766
Accession # P15056
Specificity
Detects human, mouse, and rat B-Raf . No cross-reactivity with recombinant human Raf-1 is observed. Cross-reactivity with A-Raf has not been tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat B-Raf Antibody
Detection of Human/Mouse/Rat B-Raf by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat B-Raf Monoclonal Antibody (Catalog # MAB3424) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Raf-1 (Y340E Y341E), aa 306-648 (Catalog # 4540-KS) and recombinant human B-Raf (5 ng/lane) were included. A specific band for B-Raf was detected at approximately 95 kDa (as indicated). An unidentified band at 55 kDa was also detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat B-Raf Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Sample: MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using MAB3424 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B-Raf
The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: Raf-1 (C‑Raf); A-Raf; and B-Raf, strongly expressed in fetal brain and adult cerebrum. Mice with a targeted disruption of the B-raf gene die of vascular defects during mid‑gestation. B-raf mutations have been found in two-thirds of malignant melanomas, with the single substitution V599E in the kinase domain the most frequent occurrence.
Long Name
v-Raf Murine Sarcoma Viral Oncogene Homolog B1
Alternate Names
BRaf, BRAF1, RAFB1
Gene Symbol
BRAF
UniProt
Additional B-Raf Products
Product Documents for Human/Mouse/Rat B-Raf Antibody
Product Specific Notices for Human/Mouse/Rat B-Raf Antibody
For research use only
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