Human/Mouse/Rat B7-H4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB21542
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2582H
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
Phe29-Pro258
Accession # Q7TSP5
Phe29-Pro258
Accession # Q7TSP5
Specificity
Detects mouse B7-H4 in direct ELISAs. Detects human, mouse, and rat B7-H4 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat B7-H4 Antibody
Detection of Human., Mouse, and Rat B7‑H4 by Western Blot.
Western blot shows lysates of SK-BR-3 human breast cancer cell line, mouse uterus tissue, and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat B7-H4 Monoclonal Antibody (Catalog # MAB21542) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for B7-H4 at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human/Mouse B7-H4 Monoclonal Antibody (Catalog # MAB21542) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.B7‑H4 in RAW 264.7 Mouse Cell Line.
B7-H4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse B7-H4 Monoclonal Antibody (Catalog # MAB21542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat B7-H4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP
Sample: HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Simple Western
10 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line
Sample: SK‑BR‑3 human breast cancer cell line
Western Blot
1 µg/mL
Sample: SK‑BR‑3 Human breast cancer cell line, Mouse uterus tissue, and Rat placenta tissue
Sample: SK‑BR‑3 Human breast cancer cell line, Mouse uterus tissue, and Rat placenta tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H4
Long Name
B7 Homolog 4
Alternate Names
B7H4, B7S1, B7x, Vtcn1
Gene Symbol
VTCN1
UniProt
Additional B7-H4 Products
Product Documents for Human/Mouse/Rat B7-H4 Antibody
Product Specific Notices for Human/Mouse/Rat B7-H4 Antibody
For research use only
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