Human/Mouse/Rat CrkL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5127
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 515405
Product Specifications
Immunogen
E. coli-derived recombinant human CrkL
Met1-Glu303
Accession # P46109
Met1-Glu303
Accession # P46109
Specificity
Detects human, mouse and rat CrkL in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat CrkL Antibody
Detection of Human CrkL by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CrkL Monoclonal Antibody (Catalog # MAB5127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CrkL at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat CrkL by Western Blot.
Western blot shows lysates of BaF3 mouse pro-B cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CrkL Monoclonal Antibody (Catalog # MAB5127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CrkL at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat CrkL Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line
Reviewed Applications
Read 3 reviews rated 3.7 using MAB5127 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CrkL
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein
Alternate Names
crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Gene Symbol
CRKL
UniProt
Additional CrkL Products
Product Documents for Human/Mouse/Rat CrkL Antibody
Product Specific Notices for Human/Mouse/Rat CrkL Antibody
For research use only
Loading...
Loading...
Loading...
Loading...