Human/Mouse/Rat GSK-3 beta Antibody

Detection of Mouse GSK-3 beta by Western Blot

BSSM activates AKT/GSK3 beta pathway, promotes tau hyperphosphorylation and PHF accumulation and increases the expression of active asparagine endopeptidase (AEP) and tau cleavage. (A,B) Western blotting showed higher expressions of p-AKT and the downstream p-GSK in hippocampus in BSSM treated group. (C,D) Phosphorylation of tau at S396 was higher in BSSM group than in sham and USSM group. PHF accumulation also showed significant differences between sham and the two shear stress modifier groups. (E–G) Expression of active AEP and tau N368 truncation were higher in BSSM group. (H,I) Immunofluorescent staining further reconfirmed the finding that BSSM group had more tau N368 fragments in hippocampus than the other two groups. L, left hippocampus; R, right hippocampus. *p < 0.05, **p < 0.01. Data were presented as Mean ± SD. N = 4 in each group. Scale bar, 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30337867), licensed under a CC-BY license. Not internally tested by R&D Systems.