Western blot shows lysates of human lung tissue and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HNF-3 beta/FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HNF‑3 beta /FoxA2 in Human HepG2 Cells by Flow Cytometry.

Human HepG2 cell line was stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Detection of HNF-3 beta /FoxA2 in Human iPS Cells differentiated to Mesoderm by Flow Cytometry.

Human iPS cells differentiated to Mesoderm (Catalog # SC030B) were stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

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Applications for Human/Mouse/Rat HNF-3 beta/FoxA2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HepG2 Human cell line and iPS cells differentiated to Mesoderm

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Western Blot

2 µg/mL
Sample: Human lung tissue and human pancreas tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-3 beta/FoxA2

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Entrez Gene IDs

3170 (Human)

Gene Symbol

FOXA2

UniProt

Q9Y261

Additional HNF-3 beta/FoxA2 Products

Product Documents for Human/Mouse/Rat HNF-3 beta/FoxA2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat HNF-3 beta/FoxA2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse/Rat HNF-3 beta/FoxA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2400

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all HNF-3 beta/FoxA2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat HNF-3 beta/FoxA2 Antibody

Detection of Human HNF‑3 beta/FoxA2 by Western Blot.