Human/Mouse/Rat MCHR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7938
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 851047
Product Summary for Human/Mouse/Rat MCHR1 Antibody
Immunogen
E. coli-derived recombinant human MCHR1
Ser2-Pro113
Accession # Q99705
Ser2-Pro113
Accession # Q99705
Specificity
Detects human MCHR1 in ELISAs. Detects human, mouse and rat MCHR1 in Western Blots
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat MCHR1 Antibody
Detection of Human, Mouse, and Rat MCHR1 by Western Blot.
Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MCHR1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse MCHR1 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (hypothalamus) tissue and mouse brain tissue, loaded at 0.5 mg/mL. A specific band was detected for MCHR1 at approximately 50-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human/Mouse/Rat MCHR1 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: human brain (hypothalamus) tissue and mouse brain tissue
Sample: human brain (hypothalamus) tissue and mouse brain tissue
Western Blot
1 µg/mL
Sample: Human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue
Sample: Human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCHR1
Long Name
Melanin-concentrating Hormone Receptor 1
Alternate Names
GPR24, MCH1R, SLC1
Gene Symbol
MCHR1
UniProt
Additional MCHR1 Products
Product Documents for Human/Mouse/Rat MCHR1 Antibody
Product Specific Notices for Human/Mouse/Rat MCHR1 Antibody
For research use only
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