Human/Mouse/Rat p38 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8691
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Xenograft
Applications
Validated:
Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p38 alpha
Met1-Ser360
Accession # Q16539
Met1-Ser360
Accession # Q16539
Specificity
Detects human, mouse and rat p38 alpha. Does not detect recombinant human p38 beta, p38 gamma or p38 delta.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat p38 alpha Antibody
Detection of Human, Mouse, and Rat p38 alpha by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band for p38a was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human p38 alpha by Western Blot.
Western blot shows recombinant human p38 beta, p38 gamma, p38 delta and Recombinant Human Active p38 alpha (5477-KS) (2 ng/lane). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.
Orexin B, p38a, and Integrin beta1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; MAB734), Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin beta1 Antigen Affinity-purified Polyclonal Antibody (blue; AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Applications for Human/Mouse/Rat p38 alpha Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brainstem and perfusion fixed paraffin-embedded sections of Mouse Brain Cerebellum
Sample: Perfusion fixed frozen sections of mouse brainstem and perfusion fixed paraffin-embedded sections of Mouse Brain Cerebellum
Knockout Validated
p38 alpha
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
p38 alpha knockout HEK293T cell line.
Simple Western
5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 5 using AF8691 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 alpha
Alternate Names
MAPK14, SAPK2a
Gene Symbol
MAPK14
UniProt
Additional p38 alpha Products
Product Documents for Human/Mouse/Rat p38 alpha Antibody
Product Specific Notices for Human/Mouse/Rat p38 alpha Antibody
For research use only
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