Human/Mouse/Rat PKA C alpha/ beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5908
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 515741
Product Specifications
Immunogen
E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612
Met1-Phe351
Accession # P17612
Specificity
Detects human, mouse, and rat PKA C alpha/ beta in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat PKA C alpha/ beta Antibody
Detection of Human/Mouse/Rat PKA C alpha/ beta by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PKA Ca/ beta was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human PKA C alpha/ beta by Western Blot.
Western blot shows Recombinant Human Active PKA Ca (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (1 ng/lane each). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat PKA C alpha/ beta Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA C alpha/beta
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit alpha/beta
Alternate Names
PRKACA/B
Gene Symbol
PRKACA
UniProt
Additional PKA C alpha/beta Products
Product Documents for Human/Mouse/Rat PKA C alpha/ beta Antibody
Product Specific Notices for Human/Mouse/Rat PKA C alpha/ beta Antibody
For research use only
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