Human/Mouse/Rat STAT3 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1799F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Intracellular Staining by Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 232209
Product Specifications
Immunogen
E. coli-derived recombinant human STAT3
Met1-Asn175
Accession # P40763
Met1-Asn175
Accession # P40763
Specificity
The unconjugated antibody (Catalog # MAB1799) detects human, mouse, and rat STAT3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat STAT3 Fluorescein-conjugated Antibody
Detection of STAT3 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1799F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse/Rat STAT3 Fluorescein-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: STAT3
Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. STAT3 is an 89-92 kDa, and most widely expressed, member of the STAT-family of molecules. STAT3 is considered a direct transcription factor; that is it does not act in an indirect way such as epigenetically through the regulation of DNA methylation. Activation/phosphorylation of STAT3 results in homodimerization or heterodimerization with STAT1, whereupon it enters the nucleus to principally promote cell cycle progression. The propensity of STAT3 and STAT5 toward cell division is of particular interest in oncology. Over amino acids 1-175 of STAT3 that include a protein-protein interaction domain and an NLS, human, rat and mouse show 100% amino acid sequence identity.
Long Name
Signal Transducer and Activator of Transcription 3
Alternate Names
Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)
Gene Symbol
STAT3
UniProt
Additional STAT3 Products
Product Documents for Human/Mouse/Rat STAT3 Fluorescein-conjugated Antibody
Product Specific Notices for Human/Mouse/Rat STAT3 Fluorescein-conjugated Antibody
For research use only
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