HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 PE-conjugated Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

STAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

STAT3 knockout HeLa human cervical epithelial cell line was stained with PE-conjugated Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat STAT3 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa or Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

STAT3 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT3 knockout HeLa cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT3

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. STAT3 is an 89-92 kDa, and most widely expressed, member of the STAT-family of molecules. STAT3 is considered a direct transcription factor; that is it does not act in an indirect way such as epigenetically through the regulation of DNA methylation. Activation/phosphorylation of STAT3 results in homodimerization or heterodimerization with STAT1, whereupon it enters the nucleus to principally promote cell cycle progression. The propensity of STAT3 and STAT5 toward cell division is of particular interest in oncology. Over amino acids 1-175 of STAT3 that include a protein-protein interaction domain and an NLS, human, rat and mouse show 100% amino acid sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Gene Symbol

STAT3

UniProt

P40763

Additional STAT3 Products

Product Documents for Human/Mouse/Rat STAT3 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat STAT3 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse/Rat STAT3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1799P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all STAT3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat STAT3 PE-conjugated Antibody

Detection of STAT3 in HeLa Human Cell Line by Flow Cytometry.