HEK293 human embryonic kidney cell line transfected with human S1P₅/EDG-8 and eGFP was stained with and either (A) Mouse Anti-Human S1P₅/EDG-8 APC-conjugated Monoclonal Antibody (Catalog # FAB3964A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human S1P₅/EDG-8 and eGFP and HEK293 human embryonic kidney cell line transfected with mouse S1P₅/EDG-8 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P₅ is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P₅ is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₅ show 96% and 97% aa identity with mouse and rat S1P₅, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P₅ is upregulated in large granular lymphocytic leukemias.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q9H228

Additional S1P5/EDG-8 Products

Product Documents for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3964A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all S1P5/EDG-8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse S1P5/EDG-8 APC-conjugated Antibody

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP by Flow Cytometry.