HEK293 human embryonic kidney cell line transfected with human S1P₅/EDG-8 and eGFP were stained with either (A) Mouse Anti-Human S1P₅/EDG-8 Monoclonal Antibody (Catalog # MAB3964) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P₅/EDG-8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human S1P₅/EDG‑8 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P5/EDG-8

S1P₅ is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P₅ is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P₅ show 96% and 97% aa identity with mouse and rat S1P₅, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P₅ is upregulated in large granular lymphocytic leukemias.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q9H228

Additional S1P5/EDG-8 Products

Product Documents for Human S1P₅/EDG-8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human S1P₅/EDG-8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human S1P5/EDG-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3964

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all S1P5/EDG-8 Antibodies »

Product Summary for Human S1P5/EDG-8 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human S1P5/EDG-8 Antibody

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG‑8 and eGFP by Flow Cytometry.

Applications for Human S1P5/EDG-8 Antibody